384 well plate fluorescence


1 The Plate Mate Plus instrument (Matrix) can transfer reagents from reservoirs or deep 96-well or 384-well plates into stacks of 384-well assay plates using disposable tips. When a dual-dye method was used, we found that absorbance and fluorescence gave similar results in 96- and 384-well plates. Compound transfers are performed using quilled steel pins on this instrument. o Fluorescein 2. Sensitivity ≤ 1. 25 fmol/well, 384-well plate) - top. Users can program endpoint, kinetic, multiple wavelength, linear scan and area scan measurement types. If microplate well size is interchangeable, consider investing in a microplate reader that can accommodate a multitude of plate types such as a 6- to 384-well format. View microscopic plate contents using luminescence and fluorescence with these 384-well optical bottom plates. 2ml tubes, a 96 well plate, 0. Contact a supplier or the parent company directly to get a quote or to find out a price or your closest point of sale. assay on the mirrorball® fluorescence cytometer mirrorball ® application note key benefits the purpose-built system design of TTP Labtech’s mirrorball ensures reliability for n no-wash protocol n cells and virus remain safely contained within the assay plate n compatible with high-density assay plates n whole-well scanning delivers Clear well bottoms allow for use with instruments that read above or below the plate. g. Quad monochromators (top/bottom) Sensitivity . Micro-volume (2 µL) measurements with the Take3 Plate allow dilution-free nucleic acid quantification. Instrument also comes with built-in shaker and temperature control chamber. or directly to the flat bottom of a suitable 96 or 384 well plate. 120 µl rounded square wells, flat bottom, white polystyrene White Solid Bottom Frame - low background fluorescence and minimise light w Fluorometric Assays – Fluorescence w High Content Analysis (HCA) Microplate 96-well 96 half-well 384-well 384-well low volume 1536-well Well Volume 320 to 360 µL 190 µL 125 µL 35 µL 10. This new technology can capture a full UV/vis absorbance spectrum from Elite cAMP 384-Well ELISA Kit (Fluorescence) provides a sensitive method for detecting adenylate cyclase activity in biochemical or cell-based assay system. › available with 3 different bottom shapes:F–bottom (flat), U–bottom (round), and V–bottom (conical). 384 well fluorescence plates come in black with flat bottoms. permit the reading of fluorescence-based assays STARLAB Skirted 96- and 384-Well PCR Plates feature a 15 mm high full skirt around the complete plate edge. Compared to other cAMP ELISA kits, our kit eliminates the tedious acetylation step. Fluorescein (100 nM, 25 lL/well) was dispensed into all wells of five replicate 384-well plates, and the fluorescence was read to Compatible with 6- to 384-well plates and cuvettes for assay versatility. Category Plates (53) Assay Kits (46) Electrophoresis (9) 335nm allows most fluorescence assays to be excited or read through the glass bottom. Applicable for cellular and molecular binding studies, DNA or protein quantitation, drug screening, etc. Monochromators: 250 - 700 nm . sample storage microplate / 384-well 384. 50 - 1450 µsec Fluorescence Plate Reader. The individual filter disks are encapsulated in the plate by a special over-molding process to ensure the integrity of each well. Wash cells with DPBS 1 - 2 times, and replace with 100 uL/well (for 96-well plate) or 25 uL/well (for 384- well plate) Assay Buffer (Component C). Fluorescence Supplies 384-well Microplates Our microplates are available in white for best overall well-to-well reproducibility, or black for the lowest background signal levels. …96x0. Monochromator bandwidth. 2 fmol/well 384-well plate) – Top. 5 cover glass (0. Our plates are often specified for use ThermoFisher Scientific Fluoroskan Ascent Fluorescence 96/384 Well Plate Reader model with or without injector(s) Equipped with UV-VIS Fluorescence with three year warranty $7,995. Note: When prepared aseptically, the 2X detection reagent is generally stable for 384 Well Plates. Find out all of the information about the Tecan product: fluorescence microplate / luminescence / 96-well / 384-well . Measure 96 well plate in 15 seconds or less. In addition, the Opera comes with a 10X air objective, and with 20X, 40X and 63X high numerical aperture (NA) water objectives. Wavelength range Monos: 250 - 700 nm (850 nm option) These 384-well polystyrene Nunc™ microplates are available in white, black or clear polystyrene. White walled plates enhance luminescent signals and have low background luminescence and fluorescence. Thermo Scientific Plate Guide. Fluorescein (100 nM, 25 μL/well) was dispensed into all wells of five replicate 384-well plates, and the fluorescence was read to determine the dispensing CV. 419 and 0. Reduce the volume of reagent needed with Thermo Scientific™ Nunc™ 384-Well Clear Polystyrene Plates with Cell Culture View microscopic plate contents using luminescence and fluorescence with these 384-well optical bottom plates. sample storage microplate / fluorescence / luminescence / 384-well riplate® 384. 6 – 1536-well plates, spectral scanning 6 – 384-well plates Scanning Speed: 96-well plate in 15 s, 384-well plate in 45 s, and 1536-well plate in 135 s (minimum kinetic interval time from A1 back to A1) Shaking Pattern: Orbital with adjustable speed and diameter Wavelength Selection. Since both p-values were greater than theo 384 well plate: 22 seconds . PCR plate Multiply®, with lateral skirt for Lightcycler® 480, 384 wells, white, 40 µl max. 7 to 12. These images show a portion of a 96-well plate For the 384-well plate, all other reagents used were also used at one-third the volumes used in the 96-well plate. GPCR, glow luminescence, enzyme kinetics) fluorescence and luminescence plate reader with integrated 384-well liquid handling It is equipped with four laser lines (405, 488, 561, 640 nm) for sample excitation, four high-resolution CCD cameras for fluorescence detection. 005mm), with lid, Individually packed. Detection system. run real-time kinetic cellular assays to identify early leads against GPCRs and Ion Channel targets. ADP dose response was measured with Amplite™ Universal Fluorimetric Kinase Assay Kit in a solid black 384-well plate using a Gemini fluorescence microplate reader (Molecular Devices). Many of the items listed in the tables below are available to purchase at ICCB-Longwood on an as needed basis. The top probe adjusts up and down automatically via software accommodating different plate heights, and different sized probes are available to meet specific applications. Molecular Devices Gemini XS Fluorescent Microplate Reader Specifications. The system is equipped with a fluorescence camera and a 384-pipettor head. Wavelength Accuracy < ± 1. 4 fmol fl uorescein/well, > 6 decades, 384-well plate Fluorescence intensity, bottom reading: < 4 fmol fl uorescein/well, > 5. 10-100 μl (96-well plate), 3-20μl (384-well plate) Fluorescence Chemistry: hydrolysis probes, SYBR Green I, other The Gemini XPS Microplate Reader can measure up to 4 wavelength pairs for endpoint and kinetic measurements and offers well scanning to report a fluorescent measurement from a single point in the center of a microplate well to multiple points across a tissue culture well. 384-well Plate Type Vinyl material; to seal assay plates during storage or taking fluorescence measurements Countess Automated Cell Counter Supplies. HTRF assays in HTRF 384-well plate and in Greiner 384-well plate Assay reagents were dispensed side-by-side in an HTRF 384-well low volume plate and in a Greiner 384-well low volume plate. A complete scan of a single well contains 6 x 6 stitched microscope images (tile scanning mode), taken with an objective lens 20x. 384 Well 10K 30K 100K PN 5077 PN 5076 Short Tip Long Tip 3K PN 5066 Long Tip PN 5079 PN 5078 Short Tip Long Tip PN 5081 PN 5080 Short Tip Long Tip Detection results can be further optimized by selecting the proper plate color. 5 decades, 384-well plate Time-resolved fluorescence, top reading: < 120 amol Europium/well, > 6 decades, 384-well plate 384-well plate in 45 s, and 1536-well plate What kind of 96/384 well plate should I use for fluorescence? The recommended plate is a solid black, flat bottom plate for top read. When a dual-dye method was used, we found that absorbance and five replicate 384-well plates, and the fluorescence was read to96-well plate 384-well plate 1,536-well plate 96-well plate 384-well plate 1,536-well plate 30 μL of CyQUANT® Direct reagent was added into each well. Spectral range 230-999nm for fluorescence. 419 and 0. Radius™ Gel Pretreatment Solution (Part No. Price (US dollars)* ArrayPix™ Colorimetric Microplate Microarray Scanner. 2ml strips or 39 x 0. An example of plate-to-plate dispensing variability. A complete scan of a single well contains 6 x 6 stitched microscope images (tile scanning mode), taken with an …Fluorescence Supplies 384-well Microplates Our microplates are available in white for best overall well-to-well reproducibility, or black for the lowest background signal levels. Standard 96 well-plate made of crystal-clear What kind of 96/384 well plate should I use for fluorescence? The recommended plate is a solid black, flat bottom plate for top read. luminescence and time-resolved fluorescence in a plate format with temperature-controlled environment. Optical readouts include absorbance, fluorescence intensity, fluorescence polarization, FRET, time-resolved fluorescence and luminescence. Leveraging years of assay and instrument experience in plate detection, PerkinElmer designs better Microplates for better performance that 384-Well Plates Caplugs Evergreen 384-well plates are available in both polystyrene and polypropylene. The fluorescence optics are capable of measuring all plate formats up to 384-well plates. 6- to 384-well microplate reader; Optimized with Promega cell viability and genetic reporter assays 96-well plate: less than 1 minute; 384-well plate: less 384-Well Plates 384-Well Polystyrene Plates 36 Nunc 384-Well Shallow Well Standard Height Plates 38 Cell imaging and assays, fluorescence Filter 96 well 1mL plate with filters or binding membranes PP plate with Polyethylene (PET) frit or frit plus glass fiber membranein a 96-well plate or 10,000 reactions in a 384-well plate . The well-to-well spacing is 4. The Bridge-It® L-tryptophan fluorescence assay is performed using the 96-well or 384-well microplate format and is, therefore, ideally suited for the rapid, simultaneous measurement of tryptophan in large numbers of test samples. These plates can be stored at -80 °C until needed. The BioStack is a compact and versatile microplate stacker compatible with BioTek’s washers, dispensers, detectors and imaging systems. 3. peptides, proteins, antibodies, and haptens. 384 well plate fluorescence Wells K7 and L7 were duplicate wells of 5-aza-dC. . All PCR plates are free of DNase, RNase, and human DNA. A: Corning spheroid microplates adhere to the standard ANSI/SBS footprint dimensions for 96-well and 384-well microplate formats. 384 Well, Small Volume Plate, HiBase, FLUOTRAC 600, PS, High Binding, Black, 40 Plates/Case. Briefly centrifuge small vials prior to opening. For precise quantification, technicians can 1- 384 well plates Fast scanning monochromator 200 – 1000 nm 1 nm step size Low-volume NanoQuant Plate™ At the heart of the Spark ® reader is a unique approach to fluorescence optics – each instrument gives you the capability to fully customize your optics. Catalog ID. If the measurements of FP will be corrected for background fluorescence, fill one column with protein dilution buffer alone, instead of assay solution. It offers a cost-effective, high-performance solution for reading all types of microplate formats from Envision: High speed plate reader for 96 or 384 and 1536 well plates in the following modes: UV-VIS absorbance, luminescence, fluorescence polarization, fluorescence, time resolved fluorescence assay as well as alpha screening. Ordering Information. The comparison between PHERAstar and BMG LABTECH's dedicated time-resolved fluorescence reader, RUBYstar, was performed in 96-well and 384-well formats. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. 5/μl and 30 μl was dispensed into every well of a 384 well plate. A plate dimensions guide is provided with this user manual. Sensitivity 3 pg fluorescence on each well and the emission signal is measured by a highly sensitive PMT. Molecular Devices Gemini XS Fluorescent and Fluorescence Plate Readers: Click on link to go to page ThermoFisher Scientific Fluoroskan Ascent Fluorescence 96/384 Well Plate Reader model with or without injector(s) Equipped with UV-VIS Fluorescence with three year warranty $7,995. Spectrometer-based detection The FLUOstar Omega is the fi rst multi-mode plate reader to use a CCD spectrometer for absorbance measurements. 384 Well Plates found in: Microplate Shaker, Thermal Cyclers, 384 Well storage White plates for luminescence and fluorescence assays Black plates for Black Clear Bottom Plates found in: Well, Plate, 96-Well Krystal™ Clear Bottom Polystyrene These 384-well plates have been optimized for fluorescence, Consequently, these plates are well suited for fluorescence intensity With 384-well microplates the difference in volume affects also the shape of the wells. 12, 24, 48, 96, and 384-well microplate formats, 15 sec read time for 96-well plate, comes with a computer operating on Windows 7 combining absorbance and fluorescence in 6-well to 384-well microplates, as well as endpoint, kinetic, and spectrum applications in absorbance and fluorescence using cuvettes, can be run with little to no optimization. >OptiPlates are uniquely engineered to be the same height for 96-, 384-, and 1536-well plates, and with extraordinary well flatness for ease in automation. The residual volume from 10 different 384-well microplates was determined by weighing an empty plate before and after a 3 x 300 µl per wash cycle with final aspiration. Keep cells in 100 µL/well (96-well plate) or 25 µL/well (384-well plate) of Assay Buffer II (Component C). Rounded square well design minimizes wicking and bubbling. 170±0. Polypropylene is the ideal storage plate because it has a lower binding capacity so proteins or DNA will not bind and allows for complete sample recovery. Inner well flatness < 5 µm Whole plate flatness < 25 µm. Δ Note: for 384 well plate, add 25 µL/well. Our polystyrene microplates are high binding plates, suitable for fluorescence and luminescence assays. Polystyrene is the most commonly used polymer and p olypropylene 384-well plates offer higher temperature resistance and are less reactive than polystyrene. The results of both the 96- and 384-well plate assays were counted using the ImmunoSpot ® Software (version 5. Analyzes multiple targets in 96- or 384-well plates. 5E3 10E3 10 100 1000 10000 100000 r2=0. 0 genome-wide and other DRSC libraries. The 384 Well Assay Plate. Disclaimer AAT Bioquest provides high-quality reagents and materials for research use only. Envision: High speed plate reader for 96 or 384 and 1536 well plates in the following modes: UV-VIS absorbance, luminescence, fluorescence polarization, fluorescence, time resolved fluorescence assay as well as alpha screening. Black wall plates have low background The sol-R microplate is an affordable, high-quality 384-well plate that has been designed to optimise your mirrorball ® or acumen ® fluorescence cytometer, it is equally compatible with microscope-based high content imaging devices. If a Molecular Devices LJL Analyst is used, use the rhodamine filter sets (530nm excitation filter, 590nm emission filter and 570nm dichroic mirror). Fluorescein 2. Cat. While some styles are ideal for assay testing, other compound models are primarily used for simple sample storage. Available in black or white. 96 and 384 wells; Black or white microplates; Black plates with white wells; Cell culture-treated microplates (individually wrapped) For all fluorescence applications black Microplate Reader Microplate readers, also known as microplate photometers, are instruments which are used to detect biological, chemical, or physical events of samples in microtiter plates. Fluorescence Polarization Sensitivity 1. 96 and 384 well plates (but also 6, 12, 24, 48) Option for 2 colour fluorescence (Red/Green) developed for cloning method validation experiments Screening Instrumentation Liquid Handling; It is also used to create 384-well plates from 96-well stocks (plate compression). Flipr Tetra® high-throughput cellular screening system is fast, reliable, and easy to configure. plate and 384-well plate were 0. Whole plate flatness < 25 µm High content widefield fluorescence imaging of Rat1 fibroblast nuclei, stained with DAPI (blue) in the µ-Plate 384 Well Clear. optimized for fluorescence and luminescence assays and offers user-changeable 96-, 384-, 1536-well heads. [Cytometry] Sorting into 384-well plates for single cell PCR sort 10 beads per well on flat bottom plate and verify, using a fluorescence microscope with a 20X The 384-well format assay uses <25% of the reagents recommended per well for a 96-well plate. 384 well white Measuring Time: Absorbance - 12 s (96 wells), 23 s (384 wells) Fluorescence intensity - 24 s (96 wells), 76 s (384 wells) Wavelength Range: 200 nm to 999 nm, in 1 nm increments (Absorbance) 320 to 700 nm (low noise PMT), 320 to 850 nm (red-shifted PMT) Plate Formats: 6 to 384-well platesAll Categories Sealing Films for 96-Well Microplates: AbsorbMax™ Sealing Film for Fluorescence and Photoprotection. Measurement in 384-, 1536- as well as 3456-well plate formats Simultaneous Dual Emission detection for fast and robust detection of fluorescence polarization assays, BRET, FRET and TR-FRET as well as AlphaScreen ® assays These two filter-based plate readers are capable of a variety of read modes. 384 Well Assay Microplates Thermo Scientific™ 384-Well Full Skirted PCR Plates Reduce well-to-well variability with 384-Well PCR Plate Full Skirted PCR Plates, thin walled for efficient heat transfer and compatible with most major thermal cyclers. As shown below, similar results were obtained, whether the assay was performed in Greiner plates or in HTRF plates. Wavelength selection. (A) Images from a time-lapse sequence of BS-C-1 cells migrating on a coverslip to heal a wound. • Mean Intensity Fluorescence was used to compare the formation of 96- or 384-well fluorescence plate reader-based approach, for the direct, real-time analysis of cellular respiration and mitochondrial function. An optional in-situ adapter allows for incubation of slides. The clear bottom plates offer the advantage of being able to examine the cells by microscopy during the course of the experiment. Fluorescence intensity, top reading: < 0. 384 well fluorescence plates are designed to meet industry standard dimension specifications to facilitate compatibility with microplate handling and robotics. 384-Well Microarray Microplates by Arrayit Designed for microarray manufacturing and storage, these 384-Well Microarray Microplates outperform other microplates on the market based on quality, performance, and affordability. Wavelength range. 1, 1 and 10 μM). (obtained with DELFIA reagent from PE by using a 384 well plate) Data Collection. The 384 Plus measures liquid depth and volumes down to 5µl in each microplate well and scales the microplate data to standard 1 cm cuvette values. Kinetic plate reader with an integrated dispensing head and imaging-based detector Simultaneous dispensing into the entire 96/384-well plates and simultaneous detection of the kinetics of the fluorescence Screening small-molecule compound libraries in 384- or 1536-well formats with various detection technologies (absorbance, fluorescence, luminescence, fluorescence polarization (FP), TR-FRET, AlphaScreen) Help with the use of automated instrumentation; Preparation and handling of compound libraries for high throughput screening After attaining equilibrium, fluorescence polarization (FP) signals were recorded with a CLARIOstar microplate reader (BMG Labtech, Germany) in 384-well plates (FIA plate black, 128. 88952 alamarBlue Cell Viability Assay Reagent, 100mL, • Tissue culture-compatible 96- or 384-well plates compatible with fluorometer and absorbance reading (clear or solid Remove the plate and measure fluorescence with excitation wavelength at 530-560nm and emission Corning® 96 and 384 Well Microplates with Film Bottom for High Content Imaging With an ultra-clear film, a 127 µm film thickness, and an unprecedented flatness (whole plate and intra-well), these microplates are ideal for high resolution cellular imaging applications. 384 well fluorescence plates have high binding characteristics, suitable for fluorescence assays. All new filters, parts and 3 year warranty! Instrument Description and Capabilities:Thermo Scientific Nunc 384 Well Optical Bottom Plates are ideal for microscopic applications. 230-1000nm absorbance spectral range. An Image Based Microtiter Plate Reader System for 96-well Format Fluorescence Assays Durai 96-well and 384-well wells of a 96-well plate in accord to the test The 384-well format, with its higher density of wells, allows more work to be carried out in a standard sized plate, aiding higher throughput. Methods 3D Spheroid Formation HT-1080 cells were added to wells of the 96- and 384-well PrimeSurface U bottom microplates at concentrations of 10000, 5000, 2000, 1000, 500, 250, 100, and 50 cells per well in volumes of 100 or 50 µL for the 96- or 384-well plates, respectively. BD Falcon™ 384-well TC-Treated Plates (Cell Adherence and Reproducibility) 0 20000 40000 60000 Fluorescence-based assaysPrimeSurface plates using brightfield and fluorescence imaging. Thermo Scientific Plate Guide In addition to Thermo Scientific and Thermo Scientific Nunc 96-well plates, we offer 384- and 1536-well formats You can select from a variety of optical bottom plates with superior optical properties for luminescence and fluorescence use. Polypropylene microplates are useful in drug screening/monitoring, DNA and cell biology studies. The adapter is required for optimum performance with standard 96-well and 384-96-well or 384-well microplates . The Biomolecular Analysis Facility has a Molecular Devices SPECTRAmax Gemini EM fluorescent plate reader. This kit uses 2 384-well plates that allow the measurement of about 375 samples in duplicate for either GSH or GSSG content, or measure about 183 samples in duplicate for both GSH and GSSG. pre-read plate (260 blank), the absorbance determinations at 977 nm (Test) and 900 nm (Reference), which are utilized to correct for pathlength, as well as the 260 nm experimental determination. Ideal for high-resolution imaging, sensitive fluorescence, and confocal microscopy such as single molecule detection (SMD). One column of the plate contained only buffer (background), one contained unbound AF scanning platform for reading fluorescence polarization assays. one microtiter plate with 100 μL/well in a 96-well plate, 25 μL/well in a 384-well plate, and 5 μL/well in a 1,536-well plate. Make a request. 384-well plate; Technology Platforms. The kit uses Elite™ Red as a fluorimetric substrate to quantify the HRP activity. White 384-well microplate coated with streptavidin, for use in DELFIA time-resolved fluorescence (TRF) assays. Synergy™ HTX Multi-Mode Microplate Reader is a compact, affordable system for 6- to 384-well microplates and Take3 Micro-Volume Plates. What are the advantages and disadvantages of using either type for fluorescent and luminescent experiments?Products - Microarray Substrates & Slides - Glass Bottom 96-Well and 384-Well Microplates for DNA Microarray, Protein Microarray and Whole Cell Microarray Applications Arrayit offers the market’s finest 96- and 384-well glass bottom plates activated with the most widely used microarray surface chemistries including amine and epoxy. 5 decades, 384-well plate Time-resolved fl uorescence, top reading: < 120 amol Europium/well, > 6 decades, 384-well plate Luminometry 96 well plate 90 μL 10 μL 384 well plate 36 μL 4 μL Note: Correct for background fluorescence by including control wells containing only cell culture media (no cells) on each plate. com carries 384 Well Plates from Eppendorf, Simport, Scientific Specialities, Brandtech, CAPP, Excel Scientific, Heathrow Scientific, Pipette. , uses technology similar to that of the TRF instrument in Fargoland, to perform high-throughput time-resolved fluorescence lifetime measurements in a microplate format (96 or 384 wells). Microplate Reader Application Compatibility Microplate readers by Berthold Technologies are available for fluorescence, absorbance, luminescence, TRF, HTRF®, fluorescence polarization, BRET and AlphaScreen®. Applied Biosystems OpenArray 384-Well Sample Multiplex flow cytometry on 384-well plates (~ 10 min/plate). If the read is from the bottom, a clear bottom, black walled plate should be used. 5 nM) and TTR (50 nM) in a 10 inverted question markL assay volume. 2. Bridge-It® Phosphodiesterase (PDE) Assay Kit, 384-well format The Bridge-It® Phosphodiesterase (PDE) Assay is a fluorescence-based, high-throughput screening (HTS) method for measuring cyclic nucleotide phosphodiesterase activity from a purified source and determining the IC 50 of their inhibitors. In-stock items usually ship within one business day of payment receipt. High content widefield fluorescence imaging of Rat1 fibroblast nuclei, stained with DAPI (blue) in the µ-Plate 384 Well Clear. Monitor the fluorescence signal using a fluorescence microscope with FITC filter. Handles 6, 24, 96, 384 and 1536 well plates. 384 Well Plate A clear 384 well microtiter plate with square wells and a flat, clear bottom for high throughput applications Compatible with robotics, due to an ANSI/SLAS (SBS) standard format Ideal for cell microscopy, due to the optically clear and flat bottom Excellent inner well flatness and whole plate flatness Synergy™ HTX Multi-Mode Microplate Reader is a compact, affordable system for 6- to 384-well microplates and Take3 Micro-Volume Plates. a). THE INVADER DNA ASSAY PROCEDURE – 384-WELL REACTION FORMAT The following procedure applies to assays setup manually or with automated liquid handlers. Trust BD microplates for better discovery, better Fluorescence Luminescence Radiometric Imaging of BD PureCoat amine were < 10% for 384- and 1536-well plate The 96 well μClear® microplates and 384 well μClear® microplates have a film thickness of 190 μm +/- 20 μm. Corning™ 384 Well Flat Clear Bottom Polystyrene Untreated Microplates are ideally suited for fluorescent assays using either View microscopic plate contents using luminescence and fluorescence with these 384-well optical bottom plates. 01% Triton-X100) was added to the assay buffer to avoid any false positive hits from promiscuous, aggregate-based inhibitors. Description. All new filters, parts and 3 year warranty! A black 96 well plate with square wells and flat and clear bottom for high throughput applications in cell-based assays. In the first experiment, a model system for fluorescence polarization (FP) Total fluorescence of worms dispensed into a 384 well plate is reproducible. Luminometers and fluorimeters allow for readout of luminescence (luciferase readouts) or fluorescence from an entire well of a micro-well plate such as a 96- or 384-well plate. Use a microplate heat sealer and aluminium heat seals to seal the siRNA library plates before transferring to a -80 °C for long-term storage. 4. –“To quantify SAM levels, the Bridge-It SAM fluorescence assay (Mediomics) was used according to the manufacturer’s instructions. 153 Evaluation was completed in 384 and 1536 well microplates, with both glass and polystyrene bottoms, at varying The M200PRO Multi-Detection Microplate Reader offers up to 8 detection modes such as fluorescence intensity, both UV-NIR and wavelength scanning, TRF and Luminescence measurements. It reads 6- to 384- well microplate formats including PCR trays and Terasaki plates. Analyze the microtiter plate(s) on a fluorescence plate reader using the appropriate parameters. Fluorescence intensities were normalized to DMSO alone treatment, and ICEppendorf Microplates feature the highest level of transparency compared to other polypropylene plates and therefore facilitate sample identification considerably. Fluorescence Photometric Performance. 96-well plate in 15 s, 384-well plate in 45 s, and 1536-well plate in 135 s (minimum kinetic interval time from A1 back to A1). Fluorescent Plate Reader The Biomolecular Analysis Facility has a Molecular Devices SPECTRAmax Gemini EM fluorescent plate reader. This is the ultimate tool for large groups of samples. 5E3 10E3 10 100 1000 10000 100000 r2=0. 384 well fluorescence plates are manufactured from virgin polystyrene resin. 384-well clear plate with flat bottom for colorimetric assay; black or clear 384-well plate for fluorometric assay Multi-well spectrophotometer with 384-well plate reading capability VI. 2 mP standard deviation at 1 nM fluorescein Wavelength range 280 - 700 nm (850 nm option) Time-Resolved Fluorescence Sensitivity Europium 40 fM with filters (4 amol/well in 384-well plate) Europium 1200 fM with monos (120 amol/well in 384-well plate). Luminescence, fluorescence, fluorescence polarization, time-resolved fluorescence and absorbance detection methods with 96 and 384-well plate capability. 5 For fluorescence microscope: Wash cells with Assay Buffer I (Component B) once. 340-700nm fluorescence spectral range. 384 Well Plates. 4 fmol fluorescein/well, > 6 decades, 384-well plate Fluorescence intensity, bottom reading: < 4 fmol fluorescein/well, > 5. Supplier. 1- 384 well plates Fast scanning monochromator 200 – 1000 nm 1 nm step size Low-volume NanoQuant Plate™ At the heart of the Spark ® reader is a unique approach to fluorescence optics – each instrument gives you the capability to fully customize your optics. 4 fmol fluorescein Linear Dynamic Range 6 decades 6 decades Fluorescence Intensity (FI) bottom read Wavelength Range — 300-650 nm Detection Limit – 96-well plate — 25 fmol fluorescein Detection Limit – 384-well plate — 15 fmol fluorescein Luminescence (LUM) glow 4titude®'s UltraVision™ microplates improve fluorescence-based assay results due to the ultra-clear base with superior transmission at low wavelengths. Reads 384-well microplates in 16 seconds. Equipment for Assay Development and Screening Section sub-menu with top or bottom read capabilities using 96- or 384- well plates, absorbance, luminescence, fluorescence intensity, time-resolved fluorescence and fluorescence polarization. Longer incubation times will increase sensitivity of detection. Cells/Well (384 well plate) Relative Fluorescence Units PrestoBlue TM Linearity and Sensitivity Jurkat Cells-10 min Incubation 100 400 1500 6000 2. 3 Add 100 µL/well of ROS Deep Red Working Solution into the cell plate. to 2 h in a 96-well RNAi, Oligos, Assays, Gene Editing & Gene Synthesis Tools Oligos Tools. 384-well format is the format used for RNAi reagents in the DRSC 2. Relevant Supplies . 96- or 384-well fluorescence plate reader-based approach, for the direct, real-time analysis of cellular respiration and mitochondrial function. Wash buffer was …These 96-well polystyrene microplates are designed to optimize assay performance in fluorescent applications when used with light emitting markersHTS microplates facilitate numerous tests conducted simultaneously for faster identification. Sample sizes from 5-200 ul. In general, we recommend white plates for higher-density time-resolved fluorescence assays (for example, assays run in 384-well and 1536-well plates) and …Find out all of the information about the Tecan product: fluorescence microplate / luminescence / 96-well / 384-well . Our data suggest miniaturization to 384-well format can largely keep the sensitivity and accuracy of the Luminex assays with acceptable precision. assay kit comes in a convenient 384-well Fluorescence Time Resolved Fluorescence Fluorescence Intensity top and bottom capabilities Fluorescence filters included in 1nm range Temperature control and microplate-shaking included Adjustable bandwidth range Luminescence glow reading mode 6-384-well plate capability Detail Description : 384 Well Flat Clear Bottom Black Polystyrene Poly-D-Lysine Coated Microplates, 20 per Bag, with Lid, Aseptically Manufactured Black wall plates have low background fluorescence, minimal light scatter and reduced crosstalk Amenable to 96- and 384-well microplates for high-throughput analysis on standard fluorescence plate readers (Ex/Em 380/650nm) Cell permeable probe also available for tracing intracellular O 2 levels Multiplex with MITO-ID® pH Sensor probe to confirm mitochondrial toxicity High throughput 96- and 384-well plate EliSpot/FluoroSpot analysis Designed for high throughput and traceable results. Developed with Promega luminescence and fluorescence based detection reagents, this multimode plate reader offers advanced detection capabilities in a single, easy-to-use instrument. Usable Volume: Supports a usable volume of 8. Ideal for routine fluorescence, luminescence and scintillation assays 384, 96 or 24 well format 384 well glass bottom plate. Use the following recommendations as a guideline for easy selection. The signal produced is read at 510 nm in a fluorescent plate reader with excitation at 390 nm. samples. 9948 A) Cells/Well (384 well plate) Relative Fluorescence Units PrestoBlue TM Linearity and Sensitivity Jurkat Cells--16 Hour Incubation 2 5 0 100 1000 10000 The platform is available with an integrated plate handler (Twister II) for walk-away operation (up to 160 plates). 25,000-50,000 cells/well for a 384-well poly-D lysine plate. Available in cell culture-treated, non-treated, collagen I-coated, and poly-D-lysine-coated surfaces. Ideal for fluorescence applications. The M200PRO Multi-Detection Microplate Reader offers up to 8 detection modes such as fluorescence intensity, both UV-NIR and wavelength scanning, TRF and Luminescence measurements. white (PS) – offers a high luminescence. The extreme flexibility and high sensitivity of the SpectraMax Multi- plate loader well suited for 96- and 384-well plates that can accommodate high throughput research applications. BD Falcon™ 384-well Microplates A variety of microplates suitable for a wide array of assay protocols. Plate Configuration: Plate configuration includes samples in duplicate. Eurofins MWG Operon Oligos Tool Cells/Well (384 well plate) Relative Fluorescence Units PrestoBlue TM Linearity and Sensitivity Jurkat Cells-10 min Incubation 100 400 1500 6000 2. Non-pyrogenic and has a footprint conforming to the ANSI/SBS 1-2004 Standard The BD Falcon™ 384-well Microplate product line offers a Bars represent mean signal of whole plate (384 wells). The NUNC™ Coverglass Optical Bottom 96- and 384- Well Plates have the same quality design of existing NUNC well products, plus an optically clear polystyrene plate bottom. Plate and culture cells (40 µL) in black 384-well tissue culture plates. › Come in 96 and 384 well formats. 25 fmol/well 384-well plate) – Bottom · Sensitivity Filters/mirrors: o Fluorescein 1 pM typical (0. Using a new proprietary manufacturing technique Standard Assay 384 well plates offer market leading plate flatness (± 0. Flexibility to read a variety of plate formats. The easy-to-use MitoXpress Xtra assay allows measurement of extracellular oxygen consumption rates (OCR) with whole cell populations (both adherent and The Fluorescence Lifetime Plate Reader (FLPR), designed in collaboration with Fluorescence Innovations, Inc. 384-well Plate Corning® 96 and 384 Well Microplates with Film Bottom for High Content Imaging With an ultra-clear film, a 127 µm film thickness, and an unprecedented flatness (whole plate and intra-well), these microplates are ideal for high resolution cellular imaging applications. 5 pM (0. Microplate Reader The Synergy HT Multi-Mode Microplate reader is an advanced platereader that is capable of Fluorescence, Luminescence, and UV-Visible Absorbance detection modes. 384-Well Microplates can be used with low-volume liquid handling robots used to pipette microarray printing buffers onto plates prior to microarray manufacturing Microplates can be placed on the microarrayer deck and samples loaded with four Arrayit Stealth SMP3 pins. Porvair Sciences, specialists in microplate technology, has a comprehensive range of high quality 384-well assay plates providing an ideal solution for almost all fluorescence, luminescence, absorption, ELISA/turbidity and scintillation measurements. Synergy™ HTX Multi-Mode Microplate Reader is a compact, affordable system for 6- to 384-well microplates and Take3 Micro-Volume Plates. Fluorescein 4 pM (0. Therefore, the microplates used need to be high quality and must match certain criteria. • Black 384 well plate reduces background fluorescence and minimizes backlight scatter • White 384 well plate maximizes reflection of signal while maintaining low background • Clear 384 well plate for optical clarity for readthrough applications. Save 96 well plate reader to get e-mail Tecan Genios FL TWT Fluorescence Luminescence Microplate Complete and tested 384 well fluorescent plate Figure 1: Adaptation of a wound healing assay to a 384 well plate format. TheFluorescence-based microtiter plate assay for glutamate–cysteine ligase activity. Major robotic equipment and resources used by Assay Development at the Conrad Prebys Center for Chemical Genomics include: Hamamatsu FDSS7000 rapid-kinetics (e. 384-Well Low Volume Microplates, Corning® By Corning® Plates « Back. 384 well fluorescence plates are manufactured from virgin polystyrene resin. , 24 and 48 well plates) can. ULTRA 384 Multi-Detection Microplate Reader Economical, high-performance reading of all plate formats up to 384-well microplates The ULTRA 384 from Tecan is ideal for genomics, proteomics and drug discovery applications. Pipette. PMT detector. Tecan GENios FL Reader Fluorescence Micro Plate Reader. com, product number CLS3658. All new filters, parts and 3 year warranty! Instrument Description and Capabilities:Glass Well Plate ; NARROW RESULTS BY. and black microplates for fluorescence 1- 384 well plates Fast scanning monochromator 200 – 1000 nm 1 nm step size Low-volume NanoQuant Plate™ Cuvette port: 1- 1536 well plates Fast scanning monochromator 200 – 1000 nm 1 nm step size Low-volume NanoQuant Plate™ Cuvette port ADP dose response was measured with Amplite™ Universal Fluorimetric Kinase Assay Kit in a solid black 384-well plate using a Gemini fluorescence microplate reader (Molecular Devices). 1 fmol/well 384-well plate) · …and Fluorescence Plate Readers: Click on link to go to page 24, 96, and 384 well plates for Fluorescence and Absorbance, 96 well plates for Luminescence. View microscopic plate contents using luminescence and fluorescence with these 384-well optical bottom plates. 6 ml or 1. com is your premier destination for business and facility products and services, including this PerkinElmer - 6005620 - OptiPlate-384 HB, White 384-well Microplate, Box Of 50. Incubate ≥10 minutes at 37º C. 5 decades, 384-well plate Time-resolved fluorescence, top reading: < 120 amol Europium/well, > 6 decades, 384-well plate Plate mask (aperture control) for switching between 96-well and 384-well plates Low cross-talk between wells gives you better, more usable results Broad dynamic range of the instrument extends the linear range of your assay 384 wells: 22 seconds. lasers and fluorescence detectors are used for each dye. gbo. Development of a dual luciferase-fluorescamine assay adapted to a 384 micro-well plate format-Data fluorescence units, RFU which are then converted to microgram NEST 96 Well PCR Plates NEST 96 Well PCR Plates are made from high-quality virgin polypropylene and are designed to work with all popular thermocycler machine models. The stage handles 96 and 384-well plates, up to 4 conventional slides or classical Terasaki plates. The skirt provides an extremely high plate stability, which is beneficial specifically in automated "High-Throughput" applications. Screening in the 384-well plate format and hit validation by SPR. 1. • Black 384 well plate reduces background fluorescence and minimizes backlight scatter • White 384 well plate maximizes reflection of signal while maintaining low background • Clear 384 well plate for optical clarity for readthrough applications. Legal Information Greiner high and medium binding 384 well plates clear polystyrene wells flat bottom (with micro-clear bottom), surface treatment high binding, non-sterile Synonym: 384 multiwell plates, 384 well microplates, 384 well microtiter plates, 384 well plates Achieve optimal results in cell-based fluorescence and luminescence applications using Thermo Scientific™ Nunc™ 384-Well Optical-Bottom Plate with Polymer Base or Coverglass Base, featuring a polystyrene black or white upper structure. Thermo Scientific Nunc 384-well shallow well standard height polypropylene plates provide cost savings when storing compounds for small volume applications. For protocol optimization, a gradient can be set up across the block. The 384 well microplates are available as black and white clear bottom plates (µClear®), in FLUOTRAC™, LUMITRAC™, MICROLON®, CELLSTAR®, UV-Star® or non-binding quality. reads all plate formats from 6- to 1536-well in absorbance and up to 384-well in all other detection modes. With the advancement of high-throughput flow cytometry a new tool can be added to the arsenal of cell-based screening technologies. We offer a variety of 384-well plates. It’s unique dual optics design provides superior performance for UV-Vis absorbance, fluorescence, luminescence and AlphaScreen®/ AlphaLISA® workflows. Achieve optimal results in cell-based fluorescence and luminescence applications using Thermo Scientific Nunc 384-Well Optical-Bottom Plate with Polymer Base or Coverglass Base, featuring a polystyrene black or white upper structure. The 100 µl UNIFILTER microplate is the only 384-well filter microplate with a 100 µl well volume to allow a large enough sample for recovery after filtration. Thermo Scientific Nunc 96 and. 2 mP standard deviation at 1 nM fl uorescein Wavelength range 280 - 700 nm (850 nm option) Time-Resolved Fluorescence Sensitivity Europium 40 fM with fi lters (4 amol/well in 384-well plate) Europium 1200 fM with monos (120 amol/well in 384-well plate). Corning™ 384-Well Low Flange Polystyrene Microplates have low background fluorescence, minimal light scatter and Abstract. 4titude®'s 384 well UltraVision™ microplates improve fluorescence-based assay results due to the ultra-clear base with superior transmission at low wavelengths. Time Resolved-Fluorescence Resonance Energy Transfer (TR-FRET) Assay: Representative compounds were serially diluted in dimethyl sulfoxide (DMSO) starting at 50 uM (2x starting concentration; 10% DMSO) and 10 uL were transferred into a 384-well plate. 96-well microplate / 384-well CrystalEX™ Series. In contrast to prompt fluorescence assays, 384-well OptiPlate microplate. The fastest read-times for a 96-well plate (single flash) is 20 seconds and 30 seconds for a 384 well plate. Description 384-Well Microwell Clear-Bottom Plates; White, Nontreated Surface Key Features Designed for high-volume DNA library manipulations, library and compound storage, and miniaturization of assays. 5 Monitor fluorescence increase at Ex/Em = 650/675 nm (cut White plates are ideal for luminescence and fluorescence assays, and black plates are ideal for fluorescence assays. When fluorescence data from all 384 wells on a plate/sealing material combination was analyzed, it was found that Corning plates using either reusable mats or aluminum sealing tape gave coefficient of variations of under 20%. First, the base plate and the dose response plate are pinned once using 384 well pin tool (100 nl) on pin table (Walkup pinning unit) and transferred to assay plate. One701 384) -well black-walled, clear-bottom tissue culture treated plate with each well containing Radius™ non-toxic, one biocompatible hydrogel spot (384 gel spots total per plate) 2. Sterile 8-tube strip or microplate (optional) Before reading a plate, allow the lamp in the fluorescence plate reader to warm up for at least 10 minutes and verify that the plate is aligned properly in the fluorescence plate reader. Cell Metric. com offers some of the best prices and widest selections of 384 Well Plates available. A fluorescence 96-well plate assay for GCL activity has been described by Nelson and colleagues here an assay of GCL activity that combines the sensitivity of fluorometric detection and the convenience of a 96-well (or 384-well) microtiter plate format Optimal plate height and manufacturing tolerances allow reader optics to be as close to the plate as possible, avoiding well-to-well signal crosstalk Black biochemical assay plates Intended for use in fluorescence assays, including fluorescence polarization (FP), time-resolved fluorescence (TRF) and TR-FRET applications. Natural (semi-opaque) – fluorescence, TRF White Each heat map represents one 384-well plate treated with the epigenetics library in duplicate wells at specified concentration(0. 5. Miniaturization of absorbance assays using the fluorescent properties of white microplates. 50 - 1450 µsec Designed to the standard ANSI/SLAS format – each 96-well Krystal 2000 microplate is fully compatible with all commercially available plate readers, robotic sample processors and …These 384-well opaque plates are designed for chemiluminescent and fluorescent usage for high volume laboratory work. Plates were evaluated for auto-fluorescence background, cross-talk between wells, halo effect, optical clarity,. 5ml tubes all in the same sample block. A Synergy HT configured for time- …codes 96 and 384 well filter plates feature rigid side walls of white polystyrene, making the plate ideal for automation. Fluorescence Recovery The KU-HTSL Screening facility has several plate readers to read 96, 384 and 1536 well assay plates: Biotek Synergy Neo, MicroBeta2, two SpectraMax Plus384, Gemini XS plate reader, Tecan Safire and Perkin Elmer EnVision. The optimized well design and high quality plastics provides low background and crosstalk. A black 24 well plate with flat and clear bottom for high throughput applications in cell-based assays Ideal for cell microscopy Excellent inner well flatness and whole plate flatness Low well-to-well crosstalk in fluorescence microscopyThe BD Falcon™ 384-well Microplate product line offers a variety of configurations and Bars represent mean signal of whole plate (384 wells). /bag 384 Well Lightcycler Plate - Sarstedt ab186028 Cellular ROS Detection Kit (Orange fluorescence) 9 10. Each heat map represents one 384-well plate treated with the epigenetics library in duplicate wells at specified concentration(0. 3 Add 100 µL/well of ROS Orange Working Solution into the cell plate. The 384 well microplates are available as black and white clear bottom plates (µClear®), in FLUOTRAC™, LUMITRAC™, MICROLON®, CELLSTAR®, UV-Star® or non-binding quality. Effect of Read Speed/Scan Resolution A 384 well microplate was scanned at different resolutions and the scan times were measured. Leveraging years of assay and instrument experience in plate detection, PerkinElmer designs better Microplates for better performance that Which 96 well plate would you recommend for a fluorescence assay with thioflavin T? you can use 384/96 well clear bottom black plate to apply eGFP system and curious whether it works in a The Bridge-It® L-tryptophan fluorescence assay is performed using the 96-well or 384-well microplate format and is, therefore, ideally suited for the rapid, simultaneous measurement of tryptophan in large numbers of test samples. Inner well flatness < 5 µm Whole plate flatness < 25 µm. Monitor the fluorescence increase with a fluorescence plate reader at Excitation = 540 ± 10 nm, Emission = 590 ± 10 nm (optimal Ex/Em = 540/590 nm). MatTek 384-well glass bottom plates are ideal for high-quality, high-throughput performance applications where low autofluorescence and exceptional optical clarity are needed. • Works with 96-, 384-, and 1536-well plates • Airtight sealing between -80°C and +80°C • Adhesive does not remain on the plate after peeling offFind out all of the information about the Biotek Instruments product: absorbance microplate reader / fluorescence / luminescence / with monochromator 6 - 384 well | Synergy H1. Neurotransmitter Transporter Uptake Assay 3 MATERIALS & EQUIPMENT Table 1: The Neurotransmitter Transporter Uptake Assay Kit contents Kit Components Kit Description Explorer Kit (R8173) • 5 vials of lyophilized fluorescent dye/masking dye mix, each sufficient for one (1) 96-well or 384-well plate (5 plates total). Our polypropylene multi-well plates are low binding, suitable for various assays. White 384-well microplate coated with streptavidin, for use in DELFIA time-resolved fluorescence Relevant Supplies . - Made of polypropylene to guarantee low protein absorption - Each well has a capacity of 40 µL, and can beProducts - Microarray Scanners - ArrayPix™ Microarray Fluorescence and Colorimetric Plate Scanners. HTRF assays in HTRF 384-well plate and in Greiner 384-well plate Assay reagents were dispensed side-by-side in an HTRF 384-well low volume plate and in a Greiner 384-well low volume plate. 3 mU/mL neuraminidase in a 100 µL assay volume. 384 well microplates. 50/60 Hz. Refine. An excitation/emission scan from 450nm to 550nm in 5nm increments on a 96 well plate will take approximately 150 seconds. The external dimensions of the 384 well microplates are compatible with standard equipment and automated systems. We found that plate-to-plate variability was large enough to mask the difference in optical methods. Our microplates are developed and tested to work in combination with the imaging algorithms of our multimode readers, offering assured performance. 96/384 Well Plate Reader model with or without injector(s) Equipped with UV-VIS Fluorescence with three year warranty $7,995. 384-well FLUOTRAC 200 black polystrene plate without lid, medium binding, 10/sleeve, 40/case Black plates minimize background fluorescence. All new filters, parts and 3 year warranty! Instrument Description and Capabilities: The dye was serially diluted and then transferred into a 384-well black clear-bottom plate (40 μl) for trans-absorbance mode or a low-volume, 384-well white plate (10 μl) or a 1536-well white plate (6 μl) for fluorescence quench mode. Legal Information Opaque-walled 96- or 384-well tissue culture plates are available as white or black with solid bottoms and white or black with clear bottoms. The CardioPlates are suitable for any plate-reader system and are ideal for imaging and fluorescence–based assays. The first plate had a significantly higher CV than the other four plates. Cells/Well Fluorescence (560/590nm) Figure 5. a 384-well format. Theand Fluorescence Methods for Determining Liquid compared absorbance and fluorescence methods. and black microplates for fluorescence The 96 well μClear® microplates and 384 well μClear® microplates have a film thickness of 190 μm +/- 20 μm. Save fluorescence plate reader to get e Complete and tested 384 well fluorescent plate Molecular Devices MDS FlexStation 2 384 Microplate Fluorescence Reader 2. Trans-absorbance was measured in the clear-bottom plate at 430 nm. Cell RNAi (384-well) This protocol is for RNAi screening with Drosophila cells in 384-well micro-well plates, with additional information on transfection-based RNAi in 96-well format. Nunc™ 384-Well Polypropylene DeepWell™ Storage Plate (Thermo Scientific™) Simplify compound library storage with the Thermo Scientific™ Nunc™ DeepWell™ 384-Well Plate. ), 50 pcs. This qPCR uses an Optiflex system for enhanced fluorescence detection, as well as high resolution detection for use with TaqMan Array Micro Fluidic Cards, 384 well plates and 96 well plates, as well as six decoupled excitation and emission filter channels to fully support multiplexing capabilities. It’s unique dual optics Home > Microplates > 384 Well Plates > Low Volume Plates > White Plates > Solid Bottom > 384 Well Corning Microplate, White, Small Volume, U-Bottom 384 Well Corning ® Microplate , White, Small Volume, U-Bottom Home > Microplates > 384 Well Plates > Low Volume Plates > White Plates > Solid Bottom > 384 Well Corning Microplate, White, Small Volume, U-Bottom 384 Well Corning ® Microplate , White, Small Volume, U-Bottom • In a 384-well plate the final volume in the assay is 50 µl in all of the wells. The384-Well Microplates can be used with low-volume liquid handling robots used to pipette microarray printing buffers onto plates prior to microarray manufacturing Microplates can be placed on the microarrayer deck and samples loaded with four Arrayit Stealth SMP3 pins. GFP (A) and RFP (B) fluorescence of all wells had a coefficient of variation below 9%. Pcs. 24 384 Well Shallow Well Standard Height We compared absorbance and fluorescence methods. Fluorescence. 29 Information on Corning microplates in lower density formats (e. Plate readers are very similar to spectrophotometers when measuring absorbance. We have the following plate-reader instruments available for on-site screens and other projects Fluorescence Polarization Sensitivity 1. High content widefield fluorescence imaging of Rat1 fibroblast nuclei, stained with DAPI (blue) in the µ-Plate 384 Well Clear. 4 µL for the 1536-well and 3456-well microplates, respectively. 24-, 48-, 96- and 384-well microplate formats for …Synergy™ HTX Multi-Mode Microplate Reader is a compact, affordable system for 6- to 384-well microplates and Take3 Micro-Volume Plates. Incubate the reaction for 5 to 30 minutes at room temperature, protected from light. 1 nM) makes it superior to any Opaque-walled 96- or 384-well tissue culture plates are available as white or black with solid bottoms and white or black with clear bottoms. 112502): One Sterile Bottle – 13. With the precise thermal control of the C1000 Touch CFX384 TOUCH REAL-TIME PCR DETECTION SYSTEM INNOVATIVE and detects fluorescence from The incubation took place overnight at room temperature and the final assay volumes were 100 μL in the 96-well plate, 20 μL in the 384-well plate and 7 μL in the 1536-well plate. 4-Zone™ incubation to 65°C and Condensation Control™ facilitate temperature-sensitive assays. 2 nm. Legal InformationGreiner high and medium binding 384 well plates clear polystyrene wells flat bottom (with micro-clear bottom), surface treatment high binding, non-sterile Synonym: 384 multiwell plates, 384 well microplates, 384 well microtiter plates, 384 well platesOpaque-walled 96- or 384-well tissue culture plates are available as white or black with solid bottoms and white or black with clear bottoms. These 384-well polystyrene plates conform to standard microplate footprint and dimensions. /Box . Absorbance, fluorescence, luminescence and AlphaScreen®/ AlphaLISA® measurements are all made using a unique dual-optics design that provides superior performance. 5 mm (96 well plate: 9 mm). Item Description. Detection modes include colorimetric, fluorescent intensity, fluorescent polarization, nanoBRET, time-resolved fluorescent, luminescent and AlphaScreen assays. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. The FLIPR system delivers kinetic data output from all 384 wells simultaneously to normalize the response from live cell assays. Pherastar Modules 96well plate readers use a special type of microplate to hold the samples. The high sensitivity (for as low as 0. Microplate sizes from 6- to 384-well can be read on the Gemini XPS Neobits. This contributes to lower rates of evaporation and higher data quality. All plate readers support high-throughput operations, either through stackers or via robotic integration. ThermoFisher Scientific Fluoroskan Ascent Fluorescence 96/384 Well Plate Reader model with or without injector(s) Equipped with UV-VIS Fluorescence with three year warranty $7,995. To examine the performance of the FP assay against a larger compound collection, we used the 384-well plate formatted assay in a screen of a 33,600-compound subset of the Biogen Idec 350,000 compound library. com offers some of the best prices and widest selections of 384 Well Plates available. Wavelength Bandwidth. They feature both continuous and flash lamp light sources, adjustable Z-height, with top or bottom read capabilities using 96- or 384- well plates, absorbance, luminescence, fluorescence intensity, time-resolved fluorescence and fluorescence polarization. Can be used for both top and bottom reading instruments. pcr plates, skirted, opaque, amplate, 384-well, 40ul For chemiluminescent and fluorescent procedures, the AMPLATE™-384 is available in opaque white or black. Fluorescence intensity Plate types 6- to 1,536-well plates Wavelength selection Double excitation and emission monochromators Excitation wavelength range Reads a 96-well plate in 15 sec, a 384-well plate in 45 sec, and a 1,536-well plate in 135 sec (minimum …An example of plate-to-plate dispensing variability. 0/85 mm, medium binding; Greiner Bio-One GmbH, Austria) at T = 25°C with excitation and emission filters of 485 and 520 nm, respectively. Fluorescence Intensity · Sensitivity Monochromators: o Fluorescein 2 pM typical (0. Fluorescence Supplies 384-well Microplates Our microplates are available in white for best overall well-to-well reproducibility, or black for the lowest background signal levels. The microplates range in well sizes from 6 wells up to 384 wells and sometimes even higher. The plate's upper …• Screener: 384 well and 1536 well microplates made of polypropylene Greiner Bio-One also provides various solutions for technically challenging, scientific questions requiring a parallel analysis of many samples. White plates give the highest possible signal reflection with low background fluorescence. Cor. BD Falcon™ 384-well TC Fluorescence-based The well-to-well spacing is 4. com and NEST, providing you with the opportunity to select the right 384 Well Plates for your lab. 7 ml microcentrifuge tubes . This set-up offers very good value compared to high-end HTRF readers, giving you 384-well plate throughput at a fraction of the cost. 23 384 Well Shallow Well Standard Height Plates, PS. 0 nm. 384, 96, 48, 24, 12, 6 well plates fluorescence Detection Limit – 384-well plate 3 fmol fluorescein 0. Leveraging years of assay and instrument experience in plate detection, PerkinElmer designs better Microplates for better performance that guarantees better results for all PerkinElmer HTRF assays in HTRF 384-well plate and in Greiner 384-well plate Assay reagents were dispensed side-by-side in an HTRF 384-well low volume plate and in a Greiner 384-well low volume plate. Nunclon™Δ, Poly-D-Lysine and Collagen I surface for cell culture. 2 fmol/well 384-well plate) – Top. There are no blanks or controls. 1 Combine the following in 15 mL tube: 1. calcium assay volume of 100 μL/well. Setup Guide on the BioTek Instruments FLx800 Fluorescence concentration in a 384-well plate, starting with 80 µl in column 1 and 40 µl DMSO in wells 2-20. 05, the spot size distribution is qualified as being normally distributed. The Black microplates are recommended for florescence measurements; with minimum back-scattered light and background fluorescence; while the white microplates are recommended for luminescence measurements; with maximum reflection and minimal autoluminescence. All 384-well plates are available in standard or tissue culture treated formats. Eppendorf® Microplates 96 and 384 (polypropylene) The new Eppendorf Polypropylene Microplates set new standards in terms of transparency, purity and stability! With the easy to read OptiTrack ® matrix and a clarity, unique and unbeaten for polypropylene microplates, the identification of the correct plate or an individual well is easier, less This plate reader is also a lens based fluorescence microplate reader with additional capabilities including measuring fluorescence intensity and polarization, FRET, TR-FRET, and luminescence in a range of microplates including 96- and 384-well plates. The M200Pro accommodates 6- to 384-well plates, cuvettes, and PCR plates. Synergy HTX Multi-Mode Reader Synergy™ HTX Multi-Mode Microplate Reader is a compact, affordable system for 6- to 384-well microplates and Take3 Micro-Volume Plates. The monochromator selects wavelengths in 1nm increments, and the system is equipped with a built-in programmable linear and orbital plate shaking device. BD FACSDiva™ software efficiently controls the setup, acquisition, and analysis of flow cytometry data. The HTFC Screening System discussed in this review is a platform that facilitates high-throughput flow cytometry screening in 96- and 384-well plate formats. As Basic 384-well FlashPlates contain only the scintillant, and are typically used as the base plate for coating a protein, enzyme substrate, attaching live cells, or other material of interest to build an assay upon. Well Dimensions: Afford a much lower surface area to- volume ratio compared to 384-well microplates at the same volumes. The most reliable microplate instruments for the highest performance and best results 384-well plate Fluorescence intensity, bottom reading: < 4 fmol fluorescein/well, > 5. and collection of the fluorescence occur from the top of the well. vol. We will be hoping to upscale this type of work in the near future (given the quality of the results we The CellTiter-Blue® Cell Viability Assay provides a homogeneous, Multiwell plates (96- or 384-well) compatible with fl uorescent plate readers are prepared with cells and the compounds to be tested using standard methods. Clear or white wells, bar code options. Absorbance, fluorescence, luminescence and AlphaScreen®/ AlphaLISA® measurements are all made using a unique dual-optics design that provides superior performance. 5 pM typical (0. A detergent (0. The key advantage of FLIPR over conventional plate readers is its ability to measure fluorescence emission from multiple wells (96- or 384-well) simultaneously and with high temporal resolution. Sterile 0. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex. 384 Well Assay Microplates Applied Biosystems™ MicroAmp™ Optical 384-Well Reaction Plate with Barcode Designed to provide unmatched temperature accuracy and uniformity for fast, efficient PCR amplification SpectraMax M2e is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 6-384 microplate reading capability. 96 well plate Flashes time/plate 100 1 min 7 s 50 55 s 25 48 s 10 45 s 1 42 s 1, on the fly 31 s 384 well plate Flashes time/plate 100 2 min 43 s 50 1 min 56 s 25 1 min 37 s 10 1 min 20 s 1 1 min 7 s 1, on the fly 33 s 1536 well plate meet a wide variety of assay requirements in with 6- to 384-well 384 wells: 26 seconds Fluorescence Intensity Fluorescence Test Plate The HSP90β (N-terminal) Assay Kit is designed for identification of HSP90β inhibitors using fluorescence polarization. What are the advantages and disadvantages of using either type for fluorescent and luminescent experiments? 384 Well Assay Microplates Thermo Scientific™ 384-Well Full Skirted PCR Plates Reduce well-to-well variability with 384-Well PCR Plate Full Skirted PCR Plates, thin walled for efficient heat transfer and compatible with most major thermal cyclers. A full complement of highly 6-well to 384-well microplates, 300 - 700 nm, Multi-Detection Microplate Reader, Fluorescence, Luminescence, top and/or bottom readings, 4 °C above ambient to 50 °C, 100 - 240 Volts AC. g. 384-well white polystyrene OptiPlate microplate for Time-Resolved Fluorescence (TRF), Luminescence, Alpha, 384-, and 1536-well plates, and with extraordinary well flatness for ease in automation. 384 well glass-bottom plates was compared to traditional polystyrene plates. 5 picogram fluorescein on each well or ≤4 fmol well. Storage Conditions and Reagent Preparation: Store kit at -20°C, protected from light. # Countess Cell Counting Optimal plate height and manufacturing tolerances allow reader optics to be as close to the plate as possible, avoiding well-to-well signal crosstalk. Envision: High speed plate reader for 96 or 384 and 1536 well plates in the following modes: UV-VIS absorbance, luminescence, fluorescence polarization, fluorescence, time resolved fluorescence assay as well as alpha screening. Microwell Plate Reader It is also compatible with higher density microplates such as 384-well plates. This clear base permits microscopic viewing and reading of well. 8 µL Working Volume 384 Well Assay Microplates Applied Biosystems™ MicroAmp™ Optical 384-Well Reaction Plate with Barcode Designed to provide unmatched temperature accuracy and uniformity for fast, efficient PCR amplification X500 MICROAMP OPTICAL 384-WELL REACTION PLATE The FP assay was performed in 384-well plate using very low concentration of probe 5 (1. Time-resolved fluorescence, fluorescence polarization, Alpha detection; 6- to 1536-well microplate compatible; Take3 Plate compatible for micro-volume nucleic acid quantification . qPCR Machines and Real-time PCR Platforms. com 384 well flat bottom, non-treated microtiter plate with an optimal working volume of 15-145 μL; maximum well capacity of 152 μL. Streptavidin coated DELFIA® plates bind all biotinylated biomolecules; e. 6) which includes a 384-well plate counting suite. Multiwell plates from Greiner. Since both p-values were greater than the target significance value of 0. The usable wavelengths were in the 400- to 600-nm range. Report: The report contains a matrix with: 1. Pins are washed with methanol and DMSO between each pinning. Its rapid plate exchange speeds increase throughput and enhance productivity, accommodating assay workflows for 96- and 384-well plates. RhoGEF exchange assay is suitable for measuring nucleotide exchange on GTPases in either 96 well or 384 well format. sample storage microplate. , PCR Performance Tested (free of DNA, DNase/RNase and PCR inhib. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. The excitation and collection of the fluorescence occur from the top of the well. 384 well and 1536 well microplates are largely used in automated processes with high quality standards. 4U ® CardioPlate™ 96 and 384 consist of ready-to-use, quality controlled Cor. Low well-to-well crosstalk in fluorescence microscopy; More details Surface Modification . Which 96 well plate would you recommend for a fluorescence assay with thioflavin T? you can use 384/96 well clear bottom black plate to apply eGFP system and curious whether it works in a Optical Bottom Microwell® Plates. 23 384 Well Optical Bottom Plates, PS, Coverglass Base . Products include polystyrene microplates, polypropylene microplates and UV plates. Can be used for both top and bottom reading instruments. 96- and 384-well microplate 96-well microplate / absorbance test / fluorescence test. Precision engineered and assembled, these plates are available in 96-and 384-well black matrix only for the most sensitive UV range fluorescence assays using whole-plate imaging or confocal microscopy. com carries 384 Well Plates from Eppendorf, Simport, Scientific Specialities, Brandtech, CAPP, Excel Scientific, Heathrow Scientific, Pipette. The microwellplate is housed onto a computer-controlled XY holder; it positions each well under the common termination of the fiber. o 384 well plate: 22 seconds . The white plate will increase signal output in both types of assays. riplate® 384 plate. Well volume is 120ul per well. The microplate well is part of the optical system in plate reading. com. Fluorescence Intensity · Sensitivity Monochromators: o Fluorescein 2 pM typical (0. Corning™ 384 Well Flat Clear Bottom Polystyrene TC-Treated Microplates have black wall plates for low background fluorescence, minimal light scatter, and reduced crosstalk. 384 well plate fluorescenceAchieve optimal results in cell-based fluorescence and luminescence applications using Thermo Scientific Nunc 384-Well Optical-Bottom Plate with Polymer Nunc® MaxiSorp™ 384 well plates 384 well plate, clear, polystyrene, Black 384 well plate reduces background fluorescence and minimizes backlight scatterBuy and find information on Corning 384 well plates for fluorescent assays from Sigma-Aldrich. If the read is from the bottom, a clear bottom, black walled plate …384-Well Low Volume Microplates, Corning® By Corning® Plates « Back. These high-quality NEST 96 Well Polypropylene Plates are designed for low protein absorption and minimal loss of liquid. 5 Monitor fluorescence increase at Ex/Em = 540/570 nm (cut off = 550 nm) with bottom The facility has robotic equipment for protein-based and cell-based assays using 96 or 384 well microplates. Fixed, 16 nm. 25 fmol/well 384-well plate) – Bottom · Sensitivity Filters/mirrors: o Fluorescein 1 pM typical (0. 1 The Plate Mate Plus instrument (Matrix) can transfer reagents from reservoirs or deep 96-well or 384-well plates into stacks of 384-well assay plates using disposable tips. Microwell Plate Reader with higher density microplates such as 384-well plates. 220, respectively. com and NEST, providing you with the opportunity to select the right 384 Well Plates for your lab. Products - Microarray Instruments - Microarray Microplate Centrifuges rpm and 1,500 x g centrifugation of 384-and 96-well microplates to consolidate aqueous Greiner 384 well plates, polypropylene 120 μL/well Sigmaaldrich. 4 fmol/well, 384-well plate 384-Well Plates 384-Well Polystyrene Plates 36 fluorescence Filter 96 well 1mL plate with filters or binding membranes 1-Well Format • Convenient plate For a 384-well plate, add 25 µL of H 2 O 2 cell working solution into each well instead. A Greiner low-volume, white 384-well plate was examined for fluorescence properties by scanning reader excitation and emission spectra. Wavelength range Monos: 250 - 700 nm (850 nm option) Echo® Qualified 384-Well COC, LDV Source Microplate Spectrophotometers-Fluorescence. Designed to the standard ANSI/SLAS format – each 96-well Krystal 2000 microplate is fully compatible with all commercially available plate readers, robotic sample processors and automated liquid handling systems. Ideal for cell microscopy due to black walls and optically clear flat bottom; Excellent inner well flatness and whole plate flatness; Low well-to-well crosstalk in fluorescence microscopyOpaque-walled 96- or 384-well tissue culture plates are available as white or black with solid bottoms and white or black with clear bottoms. Elite cAMP 384-Well ELISA Kit (Fluorescence) provides a sensitive method for detecting adenylate cyclase activity in biochemical or cell-based assay system. Find out all of the information about the Tecan product: fluorescence microplate / luminescence / 96-well / 384-well . Fluorescence measurements were made after 60 minutes. Monitor the fluorescence intensity with a fluorescence plate …combining absorbance and fluorescence in 6-well to 384-well microplates, as well as endpoint, kinetic, and spectrum applications in One plate carrier adapter is provided with the instrument. The BD FACSCanto II is supported by a broad portfolio of reagents. 220, respectively. 1 fmol/well 384-well plate) · Light sources Obtain superior stability and flatness with Bio-Rad Hard-Shell 384-well skirted PCR plates. This high manufacturing tolerance translates into an overall increase in measurement precision and elimination of misreads because the fluorescent imager is always focused on the cells at the bottom of each well. Take images using fluorescence microscope with a …It reduces plate read times and corrects for any signal variation due to differences in well volumes, concentrations, or fluctuations in excitation energy. 4U ® Cardiomyocytes cultured in confluent monolayers in black 96- or 384-well tissue culture plates with flat and clear bottoms. 60 fluorescence microplate / 384-well CC11 This 384-well plate is compatible with the majority of 384 block PCR Consider average sample run for choice of 96- or 384-well format. 2 Mix the 2X detection reagent well and let stand at room temperature until ready for use. Treat cells as desired: The following is an example for Hela cells treated with Foskolin to induce cAMP in a 384-well plate format. At the higher end, Molecular Devices offers a Fluorometric Imaging Plate Reader (FLIPR ®), which provides 384-well pipetting and robotic integration to increase throughput in fluorescence assays. Download document Products - Microarray Scanners - ArrayPix™ Microarray Fluorescence and Colorimetric Plate Scanners. ” TR-FRET Bridge-It® S-Adenosyl Methionine (SAM) Fluorescence Assay Kit, 384-well Protocol 850. PrimeSurface plates using brightfield and fluorescence imaging. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. • It is not necessary to use all of the wells on the plate at one time. Either white or black plates indicated for fluorescence applications can be used, Samples were measured in triplicate in 384-well white and black OptiPlate Nunc® MaxiSorp™ 384 well plates 384 well plate, clear, polystyrene, 120ul/well, pinchbar design, 30/cs; Synonym: 384 multiwell plate, 384 well Black 384 well plate reduces background fluorescence and minimizes backlight scatterBuy and find information on Corning 384 well plates for fluorescent assays from Sigma-Aldrich. Sensitivity/dynamic range Fluorescence intensity, top reading: < 0. Thermo Scientific Nunc 384-well shallow well standard height polypropylene plates provide cost savings when storing compounds for small volume applications. Product. What are the advantages and disadvantages of using either type for fluorescent and luminescent experiments? Which plates should I choose for fluorescence and luminescence measurements? I need a plate 384 Well Assay Microplates Thermo Scientific™ 384-Well Full Skirted PCR Plates Reduce well-to-well variability with 384-Well PCR Plate Full Skirted PCR Plates, thin walled for efficient heat transfer and compatible with most major thermal cyclers. 15 We set up the experiment using the ibidi µ-Plate 96 Well plates and we were able to observe perfectly the results of our experiments. Black polystyrene frame with high performance #1. The kit can detect as little as 0. Measure fluorescent intensity for each well on a fluorescence plate reader. • White plates are optimized for qPCR by increasing sensitivity and reducing variability in fluorescence detection • Cut corner: A24 Applications • PCR • qPCR/Real-Time PCR Related Products PCR Plate, 384-well, raised chimney PCR Plate, 384-well, raised chimney, barcoded, white PCR Plate, 384-well, standard, green View microscopic plate contents using luminescence and fluorescence with these 384-well optical bottom plates. Plate formats 6- to 1536- well plates, cuvettes, NanoQuant Plate Fluorescence Top reading 85 amol / well (0,85 pM, 384-well plate) FP* < 4 mP standard deviation @ 1 nM Fluorescein Prepare cells with test compounds (100 µL/well/96-well plate or 25 µL/well/384-well plate) Add equal volume of Apopxin™ Red assay solution Incubate at room temperature for 1 hour Monitor fluorescence intensity at Ex/Em = 590/630 nm (bottom read mode) Kit Key Features Non-Radioactive: No special requirements for waste treatment. The key advantage of FLIPR over conventional plate readers is its ability to measure fluorescence emission from multiple wells (96- or 384-well) simultaneously and with high temporal resolution. Each 384-well plate takes 4 x 96-well siRNA Library plates (Figure 1D). Promega GloMax Multiplus Plate Reader to run assays in a plate format (6, 12, 24, 48, 96 and 384-well). RNase-, DNase-, and human DNA-free. The Radius™ Cell Migration Assay Kit utilizes a proprietary 384-well plate to monitor the migratory DAPI Fluorescence Stain for all wells by diluting the stock Fluorescence-based microtiter plate assay for glutamate–cysteine ligase activity of a 96-well (or 384-well) microtiter plate format. The NUNC™ Coverglass Optical Bottom 96- and 384- Well Plates have the same quality design of existing NUNC well products, plus an optically clear polystyrene plate bottom. 20mm). A: Corning spheroid microplates adhere to the standard ANSI/SBS footprint dimensions for 96-well and 384-well microplate formats. Marshall Scientific has a wide assortment of microplate readers, however our most popular are the Molecular Devices Spectramax Microplate Readers. Miniaturized kinetic live-cell imaging assays 384-well plate. it is Corning CellBIND surface treated for optimal cell attachment. Offered in black or white for fluorescence assays; Available in 96-well, 384-well, and 1536-well formats; IsoPlate™ HB (high-binding) High-bind treatment for passive coating of antibodies, proteins, samples, and other biomolecules to surface of plate Clear bottom with white or black wells Suitable for bottom-reading instruments Biomat glass bottom plates are suitable for: fluorescence correlation spectroscopy (FCS), confocal imaging, fluorescence polarization (FP), cell-based assays, microarrays, laser-induced fluorescence (LIF) and cell culture. Using the automated liquid dispenser, fill an entire black opaque 384-well plate with the assay solution at 30 µl per well (this will be the assay plate). Spectral scanning speed: 2 s/well, 400 – 500 nm, 1 flash, 2 nm steps Shaking Pattern: Orbital with adjustable speed and diameter Wavelength Selection The DTX 800 features fluorescence intensity (top reading), absorbance (visible) and glow luminescence for 96- to 384-well plates, as well as linear and orbital shaking. 4 Incubate cells in a 37ºC/5% CO2 incubator for 30 – 60 minutes. Note: For a 384-well plate, add 25 μL of sample and 25 μL of assay reaction mixture into each well. The BD Falcon™ 384-well Microplate product line offers a variety of configurations and surface treatments to meet a wide array of assay needs. Cell Metric processes a single lidded microtiter plate which then removed and replaced with another one (either by hand or via a robot interface) The black wall plate has low background fluorescence, minimal light scatter and reduced crosstalk, while the optically clear flat well bottom permits direct microscopic viewing. Fluorescence Plate Reader. BD Falcon™ 384-well Microplates A variety of microplates suitable for a wide array BD Falcon™ 384-well TC-Treated Plates (Cell Adherence and Reproducibility) 0 20000 40000 60000 80000 mean RFU ± S. 9948 A) Cells/Well (384 well plate) Relative Fluorescence Units PrestoBlue TM Linearity and Sensitivity Jurkat Cells--16 Hour Incubation 2 5 0 100 1000 10000 The dye was serially diluted and then transferred into a 384-well black clear-bottom plate (40 μl) for trans-absorbance mode or a low-volume, 384-well white plate (10 μl) or a 1536-well white plate (6 μl) for fluorescence quench mode. Use the white plates for luminescence and fluorescence assays, the black plates for fluorescence assays and clear plates for colorimetric assays and sample storage. (485/535 nm) Fluorescence-based assays Cell-based assaysSolentim’s Cell Metric® is a small benchtop high-resolution whole well imaging system for cell line development and cell culture processing. Wells G11 and K11 were duplicate wells of 5-aza-C. 384 Well Optical Bottom Plates,PS, Polymer Base . Opaque-walled 96- or 384-well tissue culture plates are available as white or black with solid bottoms and white or black with clear bottoms. ab186029 Cellular ROS Detection Kit (Deep Red fluorescence) 9 10. 5 mm (96 well plate: 9 mm). According to the first aspect of the present invention, there is provided a multi-modality fluorescence reference plate comprising one or more wells, each well comprising a base, wherein the base of at least one well comprises a coating of a fluorogenic compound. D. Corning 384 and 1536 Well Microplate Selection Chart . All of these plates are manufactured from high quality polystyrene. Used for both cell- and bead-based screening. DW. SDE is the ideal tool for double-emission assays such as FRET, TR-FRET, fluorescence polarization and BRET, since the sample only has to …Equipped with a combined EliSpot/FluoroSpot module for counting and interpreting enzymatic as well as fluorescent EliSpot assays this device also comes with an automated microscope. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or spectrophotometer. 96Well Plate Absorbance. clear (PS) – for microscopic applications. Cell Metric® is a small benchtop high-resolution whole well imaging system developed for cell line development, outgrowth optimization studies and cell culture processing. The Measuring Time: 96 well plate in 15 s, 384 well plate in 45 s, 1536 well plate in 135 s (minimum times) Wavelength Range: Absorbance: 200 to 1000 nm Fluorescence intensity: EX 200 to 1000 nm, EM 270 to 840 nm Porvair Sciences, specialists in microplate technology, has a comprehensive range of high quality 384-well assay plates providing an ideal solution for almost all fluorescence, luminescence, absorption, ELISA/turbidity and scintillation measurements. Incubate the reaction at room temperature for 15 to 30 minutes, protected from light. Worms were diluted to approximately 2. 10. Black wall plates have low background Molecular Devices Gemini XS Fluorescent Microplate Reader Specifications. For a specific automated liquid handler system, follow the manufacturer’s recommendations. Microplate Reader Microplate readers, also known as microplate photometers, are instruments which are used to detect biological, chemical, or physical events of samples in microtiter plates. 0 mL 3. 32 KB. A study was conducted to determine the performance of. Additionally, the unit may be purchased with a 384 well sample block. Improve robotic handling. Take images using fluorescence microscope with a …Equipped with a combined EliSpot/FluoroSpot module for counting and interpreting enzymatic as well as fluorescent EliSpot assays this device also comes with an automated microscope. In a 96-well plate the final volume in the assay is 200 µl in all of the wells. The Infinite F200 PRO’s top reading fluorescence optics are certified for HTRF, and offer especially good performance in white, low volume 384-well plates. The non-fluorescent neuraminidase substrate becomes strongly fluorescent upon neuraminidase cleavage. Fluorescence Intensity . Greiner Bio-One is your leading global partner ▻ All information about 384 Well Microplates ▻ Contact us or buy online at shop. 5 pM typical (0. 384 Well Assay Microplates Thermo Scientific™ Nunc™ 384-Well Clear Polystyrene Plates with Cell Culture Treated and Nontreated Surfaces Reduce the volume of reagent needed with Thermo Scientific™ Nunc™ 384-Well Clear Polystyrene Plates with Cell Culture Treated and Nontreated Surfaces. The supported plate formats are 96-well, 384-well and sample slides. 75 µL and 2. Many of the detection modes (absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarization) are available stand-alone in dedicated plate readers, but are very often found today combined into one instrument (multi-mode plate reader). The easy-to-use MitoXpress Xtra assay allows measurement of extracellular oxygen consumption rates (OCR) with whole cell populations (both adherent andELISPOT Assays in 384-Well Format: Up to 30 Data Points with One Million Cells fluorescent) provides information on the numbers of polyfunctional T cells with the same sensitivity as plate and 384-well plate were 0. Riplate® 96 plates out of highly transparent polystyrene assure an optimal surface for microscopic analysis. BMG Labs PHERAstar Plate Reader. Refine your search for fluorescence microplate. Procedure using 384-well plate: 1. The instrument is located in Pinn Hall room 1074, in the Biomolecular Analysis Facility. Centrifuge the plate at 800 rpm for 2 minutes with brake off prior to the experiment. The Poly-D-Lysine and Collagen I coatings on the polystyrene plate create a positive charge on the surface that promotes cell attachment, growth, and Bmg Labtech Fluostar Optima Laboratory Lab Multi-mode Microplate Plate Reader 2 384 Microplate Fluorescence Reader Great Optiplate 384 Hb, 384-well Microplate 11